Mapipette anowanzo kushandiswa mumarabhoritari kuendesa mavhoriyamu emamililita ezvinwiwa, kubva pairi shoma kusvika pa50 ml. Mashanga anogona kuraswa mupurasitiki isina kuchena kana kushandiswazve mugirazi rinogoneka. Ose mapipette anoshandisa pipette kushuvira uye kudzinga zvinwiwa. Kukura kwakasiyana kwepipettes kunoshandiswa mukuedza kwakasiyana nepipette imwechete. Semuenzaniso, mapipetti akakosha pakusanganisa mhinduro dzemakemikari kana kumiswa kwesero, kuendesa zvinwiwa pakati pemidziyo yakasiyana, kana plating reagents pamhando dzakasiyana. Chero bedzi kutarisisa kwakabhadharwa kune vhoriyamu yemvura inoshuvira uye yakadzingwa, pipettes inogona kuva chishandiso chinobatsira murabhoritari yekufambisa nemazvo milliliter mavhoriyamu emvura.
Mhando dzepipettes uye zvakakosha zvikamu zvepipettes
Mapipettes anowanzo steriler single-use epurasitiki machubhu; anogona zvakare kuve anozvigadzirisa, anogona kushandiswazve machubhu egirazi.
Yese mapipette anoshandisa pipette kana pipetting.
Iyo pipette inobvisa kudiwa kwevanotsvakurudza kuti pipette nemuromo sepakutanga. Iyo nzira yechinyakare yepipetting haikurudzirwe nekuti ine mukana wekukonzeresa zvakakomba kuti zvinwiwa zvipinzwe mumuromo.
A pipette bhora imhando yepipette ine yakaipisisa yakarurama. Inowanzobatanidzwa negirazi pipette kuendesa huwandu hwakasiyana hwemvura.
Mapombi epipette akakodzerawo girazi pipettes, iyo inogona kuendesa mamwe chaiwo emvura mavhoriyamu. Mapombi epipette anowanzo kukodzera kugovera huwandu hwakafanana hwemvura kakawanda.
Mubatsiri pipettes ndiwo anonyanya kuzivikanwa. Inosanganisira zvikamu zvakawanda zvikuru: muromo wemuromo ndiyo iyo pipette inoiswa uye iyo iyo membrane membrane yakaiswa, iyo inodzivirira mukati memubatsiri pipette kubva pakusvibiswa kwemvura.
Mabhatani maviri anogona kuonekwa pachibato chemubatsiri pipette. Kana bhatani repamusoro rikadzvanywa, iyo mvura inoshuvira, uye kana bhatani rezasi radzvanywa, mvura yacho inoburitswa.
Mazhinji emubatsiri pipette anewo bhandi rekudzora reyero yekuyerera kwemvura. Semuenzaniso, inogona kuiswa kuti isunungure mvura iri pasi pekumanikidzwa, kana inogona kugadzirirwa kusunungurwa kwegiravhiti pasina simba rekunze.
Nepo mamwe mapipette emubatsiri achiuya netambo yemagetsi, mazhinji anofambiswa nebhatiri.
Vamwe vabatsiri pipettes vanouya nechigadziko chinopindirana munzvimbo yekubata, iyo inobvumira kuti mubatsiri pipette aiswe parutivi rwayo kana asingashandisi pasina kubvisa pipette.
Sezvambotaurwa, iyo pipette imwe chete inogona kushandisa hukuru hwakasiyana hwepipettes zvichienderana nehuwandu hunofanira kuiswa pombi, kubva padiki se 0.1 milliliters kusvika kune makumi emakumi emamiriyoni.
Basic kushanda kwepipettes
Kutanga, sarudza saizi yakakodzera pipette zvichienderana nehuwandu hwemvura yaunoda kuendesa. Wobva wavhura pasuru kubva kumusoro, bata chete chikamu chiri pamusoro pechiratidzo cheki, chiise mumuromo wepipette, uye bvisa pasuru yasara.
Zvadaro, tora pipette neruoko rumwe uye vhura chivharo chemudziyo une mvura yaunoda kushuvira. Kuchengeta pipette yakatwasuka, zvinyoro nyoro dzvanya bhatani repamusoro kuti zvishoma nezvishoma ushuvire sampuli yako.
Shandisa mutsara wapedza kudzidza pamadziro epipette kuyera huwandu hwemvura yaunoda kuendesa. Cherechedza kuti vhoriyamu inofanira kuverengwa pasi pe meniscus, kwete kumusoro.
Wobva wanyatsosunungura mvura yacho mumudziyo waunosarudza, uchichenjerera kuti usarega muromo wepipette uchibata chero nzvimbo isina mbereko.
Shandisa kuchenjerera uye simba rakapfava paunenge uchidzinga mvura, kunyanya kana uchishandisa diki vhoriyamu inokwana pipettes, kudzivirira kusvibisa mubatsiri pipette sefa uye sampuli, kana kukuvadza mubatsiri pipette. Kubata zvisina kunaka kana uchishandisa mubatsiri pipette kunogona kutsamwisa vamwe vanhu vane ruzivo murabhoritari, avo vangangotora iyo pipette parutivi kuti igadziriswe. Kana uchipomba huwandu hukuru hwemvura kana kubuda mvura, iyo yekumhanyisa yekufambisa inogona kuwedzerwa nekudzvanya bhatani zvakasimba.
Chekupedzisira, rangarira kurasa sadza nemazvo mushure mekutamisa mvura.
Application
Iye zvino zvaunoziva mashandisirwo epipette, ngatitarisei zvakadzama pane mamwe maapplication akajairwa murabhoritari.
Nhanho yakakosha kana kurima uye plating masero ndiko kugovera kwakafanana kwemaseru mumhinduro yekupedzisira. Kusimwa kwesero kunogona kusanganiswa zvinyoro uye zvinobudirira uchishandisa pipette, iyo panguva imwechete inosanganisa mishonga yemakemikari uye reagents.
Mushure mekuzviparadzanisa nevamwe kana kugadziridzwa kwemasero ekuyedza, mapipette anogona kushandiswa kutamisa maseru clones ekuwedzera kana kunotevera ongororo yekuongorora.
Nguva yekutumira: Aug-31-2022