1 The smear method inzira yekugadzira firimu rinopfeka zvakafanana zvinhu pagirazi slide.Smear zvinhu zvinosanganisira zvipenyu zvine sero rimwe chete, diki algae, ropa, bhakitiriya tsika fluid, yakasununguka matishu emhuka nezvirimwa, testis, anthers, nezvimwe.
Chenjerera paunenge uchizora:
(1) Girazi inotsvedza inofanira kuvayakachena.
(2) Siraidhi yegirazi inofanira kunge yakati sandara.
(3) Kuvhara kunofanira kuva yunifomu.Iyo smear liquid inodonhedzwa kurudyi kwepakati pesiraidhi, uye inopararira zvakafanana ne scalpel blade kana toothpick.
(4) Kupfeka kunofanira kuva kutete.Shandisa imwe siraidhi sepusher, wosaidzira zvinyoro nyoro kubva kurudyi uchienda kuruboshwe uchitevedza pamusoro pesiraidhi panodonhedzwa mushonga wesmear (kona iri pakati pemasiraidhi maviri inofanira kuva 30°-45°), woisa chidimbu chitete zvakaenzanirana.
(5) Yakagadziriswa.Nekugadzirisa, makemikari anogadzirisa kana nzira yakaoma (mabhakitiriya) inogona kushandiswa kugadzirisa.
(6) Kudhaya.Methylene yebhuruu inoshandiswa kubhakitiriya, tsvina yaWright inoshandiswa ropa, uye dzimwe nguva iodine inogona kushandiswa.Mushonga wekudhaya unofanirwa kuvhara pendi yese.
(7) Shamba.Soak yakaoma nepepa rinopinza kana toast yakaoma.
(8) Nama firimu.Nekuchengetedza kwenguva refu, simbisa masiraidhi neCanadian gum.
2. Nzira yehwendefa inzira yekugadzira mapepa nekuisa zvinhu zvehupenyu pakati pegirazi slide uye chivharo chekuvhara uye kushandisa imwe dzvinyiriro yekuparadzira masero etishu.
3. Mounting nzira inzira iyo biological zvinhu zvakanamwa sese kuita masiraidhi emhando.Iyi nzira inogona kushandiswa kugadzira makomo enguva pfupi kana asingagumi.Zvishandiso zvekutakura zvimedu zvinosanganisira: tupukanana tudiki seChlamydomonas, Spirogyra, Amoeba, uye nematodes;Hydra, epidermis yemashizha emiti;mapapiro, tsoka, miromo yezvipembenene, masero emuromo epithelial evanhu, nezvimwewo.
Kuchenjerera kunofanira kubhadharwa pakugadzirira kwemasiraidhi nzira:
(1) Paunenge wakabata siraidhi, inofanira kuva yakati sandara kana kuiswa pachikuva.Paunenge uchidonha mvura, chiyero chemvura chinofanira kunge chakakodzera, kuitira kuti chingovharwa negirazi rekuvhara.
(2) Nyaya yacho inofanira kubhedhenurwa netsono inotsemura kana kuti tweezers pasina kupindirana, uye yakatsetseka pane imwe ndege.
(3) Paunenge uchiisa girazi rekuvhara, zvishoma nezvishoma vhara mvura inodonha kubva kune rumwe rutivi kudzivirira mabhuru emhepo kubva pakuonekwa.
(4) Paunenge uchisvibisa, isa donhwe rekusvibisa mhinduro kune rumwe rutivi rwekuvhara girazi, woitora kubva kune rumwe rutivi nebepa rinonyura kuti uite mufananidzo pasi pegirazi rekuvhara rakaenzana.Mushure mekupenda, shandisa nzira imwe chete, donhedza donhwe remvura, sveta kunze kwekugadzirisa tsvina, uye tarisa pasi pe microscope.
Nguva yekutumira: Nov-22-2022